Typhoid
- Typhoid IgG/ IgM
- Typhi Sure
Typhoid Antibody Test
Typhoid test is a two-site sandwich immunoassay based on the principle of immuno-chromatography on a membrane. Each card test contains a strip composed with absorbent pad, nitrocellulose membrane, conjugate pad and sample pad. The nitrocellulose membrane is coated with test line proteins at ‘M or ‘1’ region & at ‘G; or ‘2’ region and control line protein at ‘C’ region. When a specimen containing antibody of Salmonella is applied along with assay buffer on the sample pad area, it reacts with colloidal gold conjugated with recombinant protein of Salmonella and makes an immune complex. This complex moves further chromatographically and reacts with the proteins coated on the nitro cellulose membrane leading to the formation of colored band at the (M or 1 & G or 2) region. The appearance of colored band in the specific region is considered as positive result for that particular antibody. If the specimen does not contain any salmonella / typhoid antibodies, no colored line will appear in either of the test line (M or 1 & G or 2) regions, indicating a negative test result. To serve as a procedural control, a colored line will always appear in the control line region confirming that the test has performed properly. Typhoid test is stable for 24 months from date of manufacturing if stored at 2-40°C.
Typhi Sure
Typhi Sure is a rapid test based upon lateral flow immunoassay for the qualitative and differential detection of S.typhi and S.paratyphi antigen in blood culture as in aid for the diagnosis of typhoid / paratyphoid. It is intended for in-vitro diagnosis and professional use only. Highly specific pair of monoclonal antibodies has been incorporated in the test formulation for the accurate and reliable diagnosis. Typhi Sure has 100% sensitivity for the diagnosis of S.typhi and S.paratyphi-A and100% specificity. No cross reaction noticed with blood cultures containing bacteria other than S.typhi and S.paratyphi-A. Typhi Sure has a single & simple procedural step, ensures quick & accurate results, easy to use and interpret the results without need of instrument. Use of Typhi Sure saves costs, labour and turnaround time in diagnosis of typhoid / paratyphoid. Further identification and confirmation of the bacteria in blood culture can be bypassed using Typhi Sure due to 100% sensitivity & specificity in comparison to gold standard microbiological technique. Typhi Sure is the best available tool for the resource limited settings who want to diagnose typhoid / paratyphoid without need of automated blood culture machines with the aim to minimise the time to obtain a test result, thereby allowing clinicians and pathologists to make a quick clinical decision. It has a shelf life of 24 months from date of manufacturing if stored at 2-40°C.